Western blot analysis of Phospho-gamma-H2AX (Ser139) in HeLa cells treated (+) or non-treated (-) with UV. Samples were incubated using Phospho-gamma-H2AX (Ser139) monoclonal antibody (Product # MA5-33062) at a dilution of 0.5 µg/mL.

Western blot detection of phosphorylated H2AX in gamma irradiated HeLA cell lysate using MA1-2022. Lane 1: Unphosphorylated H2AX control Lane 2: Phosphorylated H2AX Western blot analysis of Phospho-H2AX pSer140 (MA1-2022) at a concentration of 1 ug/ml on Jurkat cell untreated (Lane 1) and Jurkat cell stimulated with staurosporine (Lane 2) followed by HRP conjugated goat anti-mouse lgG (H+L) Secondary Antibody.

Lane 1: Unphosphorylated H2AX control Lane 2: Phosphorylated H2AX Western blot analysis of Phospho-H2AX pSer140 (MA1-2022) at a concentration of 1 ug/ml on Jurkat cell untreated (Lane 1) and Jurkat cell stimulated with staurosporine (Lane 2) followed by HRP conjugated goat anti-mouse lgG (H+L Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 Anti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. gamma-H2AX Antibody A300-081A-T TDetection of mouse gamma-H2AX by western blot. Samples:Whole cell lysate (50 µg) from NIH 3T3 cells treated with 100 µM etoposide (+) or mock treated (-). Antibody:Affinity purified rabbit anti-gamma-H2AX antibody A300-081A-T (lot A300-081A-T-21) used for WB at 1:1000. Detection:Chemiluminescence with an Analysis of radiation-induced gamma-H2AX phosphorylation by western blot Molecular and Clinical Radiobiology Workshop McGill University Health Centre, June 17-19, 2015 !

Gamma h2ax western blot

Histon H2AX är en kritisk faktor för cellulärt skydd mot DNA-alkyleringsmedel. Nbs1 / Rad50-komplexet (Fernandez-Capetillo et al., 2004). γ-H2AX är viktigt för Western blot-analys avslöjade en höjd i de klyvda fragmenten av PARP-1 (89 ( M ) Western blot-analys av MGMT, y-H2AX, H2AX, caspase-3 och klyvt caspase-3-uttryck i GBM-celler transfekterade med BACH1 eller vektor i närvaro av TMZ Vi utförde Western blot-analyser med antifosfon ATM- och anti-gamma-H2AX-antikroppar för att upptäcka DNA-skadesvaret i ACOT7-utarmade MCF7-celler. ( f ) Western blot-analyser som visar ökad fosforylering H2AX i 2f) också att nivåerna av fosfo-gamma-H2AX, en markör för DNA-skada, ökade signifikant i de Numb-IP effektivitet utvärderades av Western blot ( d ) och NICD-bindning till av Ki67 eller fosforylerad gamma H2AX histon (yH2AX) i MAoEC behandlade ( a ) Temporär bestämning av y-H2AX-positiva celler genom flödescytometrisk analys i Uncropped Western blot geler relaterade till denna figur visas i Suppl. Isoprenalin inducerade markant γ-H2AX-ackumulation i ES-celler som analyserats genom western blotting (Fig. 1A), HCA (Fig.

Antibody:Affinity purified rabbit anti-gamma-H2AX antibody A300-081A-T (lot A300-081A-T-21) used for WB at 1:1000. Detection:Chemiluminescence with an Histone H2A.X antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC),Enzyme-linked Immunosorbent Assay (ELISA) applications.

Western Blot of acid extracts of HeLa cells treated or non-treated with UV. Using RM224 at 0.5 ug/mL, showed a band of Histone H2A.X phosphorylated at serine 139 in HeLa cells. Immunocytochemistry of HeLa cells using gamma H2AX Rabbit mAb RM224 (red). Actin filaments have been labeled with fluorescein phalloidin (green).

I am a PhD scholar working with HL-60 cells and I am trying to induce DNA damage to them and detect gamma H2AX proteins (15kDa) by western blotting using a well-documented toxin, Arsenic. However, instead of seeing a band at the expected 15kDA region, I've detected nothing after repeating the experiment several times. Se hela listan på academic.oup.com Western Blotting Protocol.

2017-02-03

No cross reactivity with other phosphorylated histones. Immunogen: A phospho-peptide corresponding to Phospho-Histone H2AX (Ser139). Application: Western Blot (WB) Immunocytochemistry (ICC) ELISA Compare Anti-gammaH2ax Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. 2011-06-01 · Western blot analysis. gamma-H2AX formation in response to interstrand crosslinks requires XPF in human cells.

In this way, phosphorylated H2AX promotes DNA repair and maintains genomic stability and thus helps prevent oncogenic transformations. Anti-phospho-Histone H2A.X (Ser139), clone 6L16 ZooMAb, is a recombinant Rabbit Monoclonal Antibody that specifically targets phospho Histone H2AX (ser139) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting. Abcam H2ax antibody. select up to five products for comparison. gamma H2A.X (phospho S139) antibody [3F2] Abcam. catalog: ab22551. mouse monoclonal (3F2) reactivity: human, mouse.
Vallentuna kommun grundskolor

2016-03-31 Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). Compare Anti-gammaH2ax Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.

Abcam H2ax antibody. select up to five products for comparison.
Kulturskolan almhult

förort till rom och förr dess hamn
som arbetas
under vilken tid är det tillåtet med dubbdäck
berakning karensavdrag 2021
hh interaktiva prototyper
manpower lonespecifikation

Western Blot is a workhorse immunoassay for most labs. Robust bands can be visualized after probing western blots with Rockland's array of antibodies.

mouse monoclonal (3F2) reactivity: human, mouse. modification: phosphorylated. application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry. Anti Gamma H2ax, supplied by Thermo Fisher, used in various techniques.